Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Peter Nguyen
Date created: 2006-10-24 11:00:00
Date modified: 2015-08-31 03:54:04
ComA
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_J51001_sequence (Version 1) |
Description
ComA is the reponse regulator of the ComQXPA quorum-sensing system of Bacillus subtilis. In the presence of extracellular ComX pheromone (a secreted 10-residue modified peptide), ComP binds to ComX and activates ComA by phosphorylation. ComA-p then binds to specific promoter sequences and activated transcription of a number of genes.NOTE: The ComA part described herein contains an internal SpeI site, which prevents cloning using standard assembly.
For information on the DNA binding sequence for ComA can be found in:
J. Bacteriology (1993) 175(10), 3182-3187.
For details about the ComQXPA system of quorumtaxis, see:
Molecular Microbiology (2005) 57(4), 1159???1174.
Notes
NOTE: The Biobrick prefix and suffix sites are non-standard! Due to the high percentage of A+Ts in the ComQXPA genes, the primer hybridization sites were lengthened and the Biobrick sites correspondingly shortened (the NotI sites between the E-X and S-P restriction sites was removed).Primers used for sequencing (5'->3')
F: cggaattccctctagATGAAAAAGATACTAGTGATTGATGACCATCCGG
R: gactgcagctactagTTAAAGTACACCGTCTGATTTCGCAATC
NOTE: The ComA part contains an internal SpeI site, which prevents cloning using standard assembly. This will be removed by site-directed mutagenesis in the future.