Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jeffrey J Tabor
Date created: 2007-04-02 11:00:00
Date modified: 2015-06-15 12:42:46
Medium copy ColE1 BioBrick vector
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_J64100_sequence (Version 1) |
Description
This plasmid is based on pProTetE333 (clontech). A SpeI site was removed via an A-T mutation at position 1166 on the original plasmid, resulting in pProTetE333b. The G00100-G00101 fragment of pSB4A3-1 which includes P1010 between a biobricks multiple cloning site which itself is flanked by + strand transcriptional terminators was then cloned between the XhoI and AvrII sites of pProTetE333b. The resulting plasmid carries a ColE1 origin of replication, a Chloramphenicol resistance marker, the biobricks MCS and the ccdAB death cassette.Notes
NoneSource
pProTetE333 (clontech) and biobricks parts| Sequence Annotation | Location | Component / Role(s) |
| G00100 prefix P1010 suffix G00101 ColE1 origin chloramphenicol resistance marker G00101 | 6,25 124,145 146,820 821,841 977,996 1130,1812 1939,2598 1029,1048 | primer_binding_site feature/primer_binding feature/BioBrick engineered_region engineered_region feature/BioBrick feature/BioBrick engineered_region primer_binding_site feature/primer_binding sequence_feature feature/misc feature/misc sequence_feature feature/primer_binding primer_binding_site |