Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julie Norville
Date created: 2009-08-16 11:00:00
Date modified: 2015-05-08 01:08:23
yfp RBS, {0,5
15,10} family member - B0032 simulator (reverse oligo)
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_J70461_sequence (Version 1) |
Description
These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;5,0})-YFP to (Promoter X)-(RBS level Y)-YFP.Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example
Also gtg starts are removed to make the RBS more predictable.
Form of insert:
{promoter} ta ctaga ^ g {rbs master sequence} a tgagc ^ {rest of YFP (E0030)}
{promoter} at ^ gatct c {rbs master sequence} t^ actcg {rest of YFP (E0030)}
Forward oligo:
g {rbs master sequence} a tgagc
Reverse oligo:
Reverse complement of (ctagag {rbs master sequence} a)