BBa_J70465

BBa_J70465 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J70465
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julie Norville
Date created: 2009-08-16 11:00:00
Date modified: 2015-05-08 01:08:23

yfp RBS, {0,5
15,10} family member - B0034 simulator (reverse oligo)



Types
DnaRegion

Roles
Other

sequence_feature

Sequences BBa_J70465_sequence (Version 1)

Description

These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;5,0})-YFP to (Promoter X)-(RBS level Y)-YFP.

Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example B0034.)

Also gtg starts are removed to make the RBS more predictable.

Form of insert:
{promoter} ta ctaga ^ g {rbs master sequence} a tgagc ^ {rest of YFP (E0030)}
{promoter} at ^ gatct c {rbs master sequence} t^ actcg {rest of YFP (E0030)}

Forward oligo:
g {rbs master sequence} a tgagc

Reverse oligo:
Reverse complement of (ctagag {rbs master sequence} a)

Notes

none

Source

designed

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J70465/1