BBa_J70535

BBa_J70535 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J70535
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julie Norville
Date created: 2009-12-08 12:00:00
Date modified: 2015-05-08 01:08:24

gfp RBS, {0,5
15,10} family member - B0032 simulator (reverse oligo)



Types
DnaRegion

Roles
Temporary

sequence_feature

Sequences BBa_J70535_sequence (Version 1)

Description

These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;15,10})-GFP to (Promoter X)-(RBS level Y)-GFP.

Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)

(In this case it was not necessary to remove gtg starts since they were not present near the start of gfp.)

Form of insert:

{promoter} ta ctaga ^ g {rbs master sequence} atg c gtaaa ^ {rest of GFP (E0040)}

{promoter} at ^ gatct c {rbs master sequence} tac g^ cattt {rest of GFP (E0040)}

Forward oligo:

g {rbs master sequence} atg c gtaaa


Reverse oligo:

Reverse complement of (ctagag {rbs master sequence} atg c)

Notes

BioScaffold insert

Source

These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;15,10})-GFP to (Promoter X)-(RBS level Y)-GFP.

Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)

(In this case it was not necessary to remove gtg starts since they were not present near the start of gfp.)

Form of insert:

{promoter} ta ctaga ^ g {rbs master sequence} atg c gtaaa ^ {rest of GFP (E0040)}

{promoter} at ^ gatct c {rbs master sequence} tac g^ cattt {rest of GFP (E0040)}

Forward oligo:

g {rbs master sequence} atg c gtaaa


Reverse oligo:

Reverse complement of (ctagag {rbs master sequence} atg c)

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J70535/1