BBa_J70592

BBa_J70592 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J70592
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Lynch
Date created: 2010-06-17 11:00:00
Date modified: 2015-05-08 01:08:25

RFC12 Constitutive Promoter



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_J70592_sequence (Version 1)

    Description

    This is a RFC12 compatible version of part BBa_J23100. It is a constitutive promoter isolated from a small combinatorial library.

    Notes

    Designed with synthetic oligos:

    5' AATTC GCGGCGC T ACTAGT TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC GCTAGC A GCGGCCG CTGCA 3', Prom-F
    5' GCGGCCGCTGCTAGC GCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAA ACTAGTAGCGCCGCG 3', Prom-R
    Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

    Source

    BBa_J23100

    Sequence Annotation Location Component / Role(s)
    -35
    -10
    		1,6
    23,28
    		feature/misc sequence_feature
    sequence_feature feature/misc

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J70592/1
     

    Attachments

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