Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26
{Bmll56} in BBb
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_J72016_sequence (Version 1) |
Description
{rbs_at_1000.6XHis} RBS with an N-terminal 6XHis tag.Notes
N/ASource
Overlap Extension PCR (Wobble construction)Wobble construction of Bmll56 {rbs_at_10000.6XHis>}
Wobble mll56/mll57 (88, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll56 {rbs_at_10000.6XHis>}
mll56 Forward wobble for {rbs_at_10000.6XHis>} (Bmll56)
CCATAGAATTCATGAGATCTCTACAACACCTAGCATCTAAGAAGTTACATATGGATTC
mll57 Reverse wobble for {rbs_at_10000.6XHis>} (Bmll56)
CGTTAGGATCCGTGATGGTGATGATGGTGAGAATCCATATGTAACTTCTTAG
| Sequence Annotation | Location | Component / Role(s) |
| 6XHis tag Rbs_at_1000 | 45,60 6,36 | feature/cds CDS feature/rbs ribosome_entry_site |