BBa_J72030

BBa_J72030 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J72030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Brophy

Date created: 2010-07-14 11:00:00

Date modified: 2015-05-08 01:08:26

{Bmll62} in BBb

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_J72030_sequence (Version 1)

Description

{E!} C terminal E tag.

---

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll62 {

Wobble mll62/mll63                (73, EcoRI/BamHI

Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-Bmll62         {


mll62    Forward wobble for {
CCATAGAATTCATGAGATCTGGTGCTCCGGTTCCATATCCAGATCCACTG

mll63    Reverse wobble for {
CGTTAGGATCCTTAACGTGGTTCCAGTGGATCTGGATATGG

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
E tag	6,45	CDS, feature/cds

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J72030/1

Member of these Collections

• iGEM Parts Registry