BBa_J72037

BBa_J72037 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J72037

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Brophy

Date created: 2010-07-14 11:00:00

Date modified: 2015-05-08 01:08:26

{Bmll5} in BBb

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_J72037_sequence (Version 1)

Description

{Med7DNDc102-205!} Protein 7 of the Mediator Complex (MED7) with a stop codon. This version of MED7 was made to be tagged N-terminally.

---

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

PCR
Construction of MED7 Basic Part Bmll5
PCR MLL3/MLL78 on pBca9523-Bmll3 (324bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+910, L)
Product is pBca9523-Bmll5
-------------------------------------
MLL3 Forward Biobricking of Bmll5 CCATAGAATTCATGAGATCTATGAAAAAATCTACCGAAA
MLL78 Reverse Biobricking of Bmll5 CGTTAGGATCCAGAGGTCAGTTTGTCGTGAACCTGTTTG

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J72037/1

Member of these Collections

• iGEM Parts Registry