BBa_K081004

BBa_K081004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K081004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lorenzo Pasotti, Paolo Magni
Date created: 2008-10-18 11:00:00
Date modified: 2015-05-08 01:08:34

Demux SEL



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K081004_sequence (Version 1)

Description

This composite part is the union of:
*a PoPS in -> cI, lasR out device and
*a Plambda-regulated luxR protein generator with Plux downstream.

It is a module of the following genetic demultiplexer (demux):
{|align="center"
|[[Image:Pv_demux_final.png|500px]]
|-
|[[Image:pv_demuxregistry_interactions.png|500px]]
|}
where:
*PA, PS and PB are three promoters that can be ACTIVATED by the three generic elements A, S and B respectively;
*GOI is the gene of interest that is the output of the system.

In particular, K081004 is the Selector.

People who want to use the genetic mux have to assemble:
*K081000 under the desired regulation for Channel 0 (for example the generic promoter PA);
*K081001 under the desired regulation for Channel 1 (for example the generic promoter PB);
*K081002 under the desired regulation for Selector (for example the generic promoter PS);
*the desired protein generator downstream of K081000 and K081001 (for example the generic RBS-GOI-T generator);

Notes

We used BioBrick Standard Assembly.

Source

a

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Works
 
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It's complicated
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Planning
 
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BBa_K081004/1