BBa_K090501

BBa_K090501 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K090501

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Goodman

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:08:37

Gram-Positive IPTG-Inducible Promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K090501_sequence (Version 1)

Description

This is an IPTG-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.

Notes

We found the functional annotation in the associated paper:
Kaltwasser M., T. Wiegert, and W. Schumann. 2002. Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis. Appl. Environ. Microbiol 68:2624-2628

The primers we used to extract it (sans biobrick ends) are as follow:
Forward: AGAACAACCTCTGCTAAAATTC
Reverse: AAGCTTAATTGTTATCCGCTC

Source

We PCRed this part out of the pMUTIN-YFP gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE151.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35	43,48	feature/promoter, promoter
-10	66,71	promoter, feature/promoter
lacOid Operator	78,97	operator, feature/operator

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K090501/1

Member of these Collections

• //chassis/prokaryote/subtilis
• //direction/forward
• //promoter
• //regulation/negative
• //rnap/prokaryote/subtilis/sigmaa
• iGEM Parts Registry