BBa_K090506

BBa_K090506 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K090506

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Goodman

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:08:37

Bacillus subtilis weak RBS

Details

• Types: DnaRegion
• Roles: ribosome_entry_site; RBS
• Sequences: BBa_K090506_sequence (Version 1)

Description

This is the weak version of the consensus RBS for Bacillus subtilis. The sequence is present in front of some B. subtilis operons, but is expected to give weaker expression than the consensus sequence.

The consensus sequence AAAGGAGG (BBa_K090505) was changed to AGAGGTGG for this part.

Notes

The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AGAGGTGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.

Source

This part was synthesized directly.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
B. subtilis weak RBS	1,8	feature/rbs, ribosome_entry_site

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K090506/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //direction/forward
• //rbs/prokaryote/constitutive/miscellaneous
• //regulation/constitutive
• //ribosome/prokaryote/bsubtilis
• iGEM Parts Registry