LacI_I12

BBa_K091122 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K091122

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pallavi Penumetcha

Date created: 2008-07-08 11:00:00

Date modified: 2015-05-08 01:08:38

LacI_I12 protein

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K091122_sequence (Version 1)

Description

This is a variation of the LacI protein that acts as a repressor for the pLac promoter and is repressed by IPTG. It has a point mutation at the third codon where proline is replaced by tyrosine. This missense mutation causes an increased binding affinity of the LacI_I12 protein with the pLac promoter.

Notes

Because there are many codons that code for the amino acid tyrosine, we chose to use the TAT codon, because it was the most common tyrosine codon found throughout the rest of the LacI wild-type gene sequence.

Source

The basic sequence comes from Ecogene.com

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Proline->Tyrosine	7,9	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K091122/1

Member of these Collections

• //cds/transcriptionalregulator/repressor
• iGEM Parts Registry