BBa_K091151

BBa_K091151 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K091151

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erin Feeney

Date created: 2008-07-17 11:00:00

Date modified: 2015-05-08 01:08:38

pLas' Testing Construct D

Details

• Types: DnaRegion
• Roles: engineered_region; Signalling
• Sequences: BBa_K091151_sequence (Version 1)

Description

This part contains a constitutively active promoter, rbs, LuxR coding region, and LasR coding region followed by the pLas' promoter and GFP. K091151 generates both LasR and LuxR to test crosstalk and competitive binding with the pLas' promoter in the presence of AI-1 and PAI-1.

Notes

pLas' Testing Construct D has the coding region for LuxR preceding the region for LasR, but the order of these regions is reversed in pLas' Testing Construct C

Source

The sources of the quorum sensing components are the modified lasI regulatory region in P. aeruginosa and the lux components from V. fisheri.

Components

Access	Instance	Definition
public	BBa_S01640	BBa_S01640
public	BBa_S03954	lasR
public	BBa_S03984	BBa_S03984

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_S01640	1,799	BBa_S01640
BBa_S03954	808,1743	lasR
BBa_S03984	1752,2761	BBa_S03984

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K091151/1

Member of these Collections

• iGEM Parts Registry