BBa_K092700

BBa_K092700 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K092700

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: EPF-Lausanne Team 2008

Date created: 2008-10-18 11:00:00

Date modified: 2015-05-08 01:08:40

TetR cds with RBS and Terminator + pTetR, RFP with RBS + LuxI with RBS and terminator

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K092700_sequence (Version 1)

Description

Combination of the following parts : BBa_K092000, BBa_K092300 and BBa_K092400:
The TetR sequence (with a ribosome binding site and terminator)[BBa_K09200] is bound to the part containing the promoter of TetR and the red fluorescent protein (RFP) with a ribosome binding site. A luxI part (with RBS and terminator) is added at the end of the cds of the RFP. As no terminator was placed after the RFP, they both depends on the TetR promoter. The transcription of RFP and LuxI is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.

Components

Access	Instance	Definition
public	BBa_K092600	BBa_K092600
public	BBa_K092400	LuxI

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K092600	1,1634	BBa_K092600
BBa_K092400	1643,2414	LuxI

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K092700/1

Member of these Collections

• iGEM Parts Registry