Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julian Wiegelmann, Danielle Nash
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:08:40
pRecA with LexA binding site
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K093000_sequence (Version 1) |
Description
This is a pRecA promoter with LexA binding sites. LexA is cleaved by RecA when RecA is upregulated due to induction of the SOS response triggered by single stranded DNA. For this part to work it must be in a RecA+ strain of E. coli such as TG1Notes
How far past the start site of transcription do you need to go in order for the promoter to drive transcription? The promoter has been confirmed to drive transcription in a regular DH5alpha strain, so it the answer to the question is, 3 bp before the suffix, maybe less.The LexA binding site is in the core region of the promoter (between the -35 and -10 sigma 70 binding sites). According to Robert Sidney Cox III and colleagues in their paper "Programming ene expression with combinatorial promoters, the core region is the most effective region for binding of repressor molecules.