BBa_K098988

BBa_K098988 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K098988

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Harvard iGEM 08

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:08:41

temperature sensitive cI inducible system with GFP reporter and high promoter

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K098988_sequence (Version 1)

Description

This is a temperature sensitive cI inducible system driven by a high promoter (BBa_J23114) for the repressor and with a GFP reporter. It can be used to regulate the expression of GFP using heat.

Notes

This is a mutagenized form because the original product had an extra PstI site between the repressor and terminator. While there are still extra basepairs between the end of the repressor sequence and the Biobricks scar, they no longer code for a PstI site.

The extra base pairs are: [repressor coding region] CTCCAGCGGCCGC [Biobricks scar]

Source

PGW7 plasmid and Biobricks parts.

Components

Access	Instance	Definition
public	BBa_J23114	BBa_J23114
public	BBa_S04138	BBa_S04138

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23114	1,35	BBa_J23114
BBa_S04138	44,1813	BBa_S04138

Other Properties

• igem#experience: Works
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K098988/1

Member of these Collections

• iGEM Parts Registry