Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sarah Hendrickson
Date created: 2008-07-27 11:00:00
Date modified: 2015-05-08 01:08:41
TetR and p22 cII Dual Promoter Driving RFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K101007_sequence (Version 1) |
Description
This is a construct composed of a dually-repressed promoter, an RBS of medium strength, the reporter protein RFP, and a single, well-characterized terminator. The promoter is repressed in the presence of TetR and/or p22 cII, and ideally allows no expression of the reporter RFP. The RFP used is BioBrick part J06504, a monomeric RFP which has been optimized for bacteria.The presence of RFP makes this construct a useful reporter in E.coli systems in which expression levels of either TetR and/or p22 cII need to be examined.
Notes
During the design of this part, the majority of design considerations were dealt with during the construction of the TetR/p22 cII dually repressed promoter (K101002). The promoter design required careful consideration of which operator sites to include, as well as which -35 and -10 sites should be utilized.A medium strength RBS was chosen to increase the ribosome binding affinity to an appropriate level. Additionally, the decision was made to use only a single, well-characterized terminator, rather than a double terminator.
Source
Constructed from genomic sequences obtained from previously designed BioBrick parts.| Access | Instance | Definition |
| public public public public | BBa_K101002 BBa_B0032 BBa_J06504 BBa_B1006 | BBa_K101002 BBa_B0032 mCherry BBa_B1006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K101002 BBa_B0032 BBa_J06504 BBa_B1006 | 1,66 75,87 94,807 816,854 | BBa_K101002 BBa_B0032 mCherry BBa_B1006 |