BBa_K1021032

BBa_K1021032 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1021032

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Swati Sureka

Date created: 2013-09-14 11:00:00

Date modified: 2015-05-08 01:08:44

PT7+nptII

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K1021032_sequence (Version 1)

Description

The neomycin phosphotransferase gene (nptII), which also confers resistance to geneticin, sits behind the T7 promoter.

Notes

An RBS was excluded because this part was designed for T7-based expression in fungi.

Source

The promoter comes from the T7 virus; the gene, originating from E. coli K12, was taken from the pNG transformation vector.

Components

Access	Instance	Definition
public	BBa_I712074	BBa_I712074
public	BBa_K1021001	BBa_K1021001

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I712074	1,46	BBa_I712074
BBa_K1021001	53,853	BBa_K1021001

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1021032/1

Member of these Collections

• iGEM Parts Registry