Si4

BBa_K1028000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1028000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Oleg Karpov, Joseph Beton

Date created: 2013-08-29 11:00:00

Date modified: 2015-05-08 01:08:46

Silica binding peptide

Details

• Types: DnaRegion
• Roles: tag; Tag
• Sequences: BBa_K1028000_sequence (Version 1)

Description

This is a short peptide of 15 amino acids designed to bind with high affinity to silica and silica-like particles, designed as a tag to be attached to the C-terminus of polypeptides. This part was originally designed to be conjugated to the C-terminal of Ice Nucleation Protein allowing extracellular expression of the binding region.

Notes

The sequence was codon optomised for E.Coli expression.

Source

This sequence is taken from the "Site-Specific Labeling of Surface Proteins on Living Cells Using Genetically Encoded Peptides that Bind Fluorescent Nanoparticle Probes" paper published by Rocco et al.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
stop codon	46,46	feature/stop, stop_codon
stop codon	49,49	feature/stop, stop_codon
Coding region	1,45	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1028000/1

Member of these Collections

• iGEM Parts Registry