Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei Huang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:46
Zeo-resistant gene + GFPuv
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1030008_sequence (Version 1) |
Description
We use a zeocin resistant gene and external zeocin to tune the cell growth rate. The common antibiotic resistant genes are reserved for plasmid backbones. GFPuv is used to monitor the expression of zeocin resistant gene. In addition, zeocin resistant gene allosterically inhibitor the effect of zeocin, makes it easy to be quantified and mathematically modeled.Notes
We want to use signalling molecule produced by E coli to tune zeo-r expression, and its growth rate withzeo presents.Source
Zeo is from a commercial plasmid. GFPuv is from iGEM| Sequence Annotation | Location | Component / Role(s) |
| GFP protein BBa_E0040 | 1,720 1,720 | CDS feature/protein feature/BioBrick engineered_region |