BBa_K1031911

BBa_K1031911 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1031911

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: He Shuaixin

Date created: 2013-08-31 11:00:00

Date modified: 2015-05-08 01:08:48

XylS-Terminator

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1031911_sequence (Version 1)

Description

XylS senses benzoate and its substitutes, it is the transcriptional activation factor that activates the expression of meta-operon for benzoate degradation on TOL plasmid pWW0 in Pseudomonas putida.
This part contains XylS coding sequence with terminator. To complete the sensor, please add promoter before XylS coding sequence.

Notes

Molecular cloning to add terminator to XylS coding sequence.

Source

XylS comes from Pseudomonas putida, sequence found through Genbank: http://www.ncbi.nlm.nih.gov/nuccore/M10143.1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ATG start coden	1,3	start_codon, feature/start
XylS CDS	1,966	feature/cds, CDS
B0015	974,1103	feature/BioBrick, engineered_region
TGA stop coden	964,966	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1031911/1

Member of these Collections

• //cds/transcriptionalregulator/activator
• //chassis/prokaryote
• iGEM Parts Registry