Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zheng Pu
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:08:48
Pm promoter
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1031921_sequence (Version 1) |
Description
The cognate promoter regulated by XylS, Pm, is σ70-dependent in E.coli; meanwhile in Pseudomonas putida, it is σ54-dependent. It acts as the master regulator to control the ON/OFF expression of meta-operon on TOL plasmid pWW0. In this meta-operon, XylXYZLTEGFJQKIH genes encode enzymes for the degradation of benzoate and its derivatives, generating intermediate products in TCA cycleNotes
Pm promoter+ eGFP, we didn't use sfGFP because the intensity of eGFP is more suitable for our biosensor.Source
Pseudomonas putida, TOL pWW0 plasmid| Access | Instance | Definition |
| public | BBa_E0840 | GFP genera |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_E0840 | 1,878 | GFP genera |