BBa_K1039006

BBa_K1039006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1039006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Urooj Kishor

Date created: 2013-09-09 11:00:00

Date modified: 2015-05-08 01:08:50

Bxb1 Recombination Directionality Factor

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1039006_sequence (Version 1)

Description

This is the recombination directionality factor (RDF) that acts with Bxb1 serine integrase. It reverses the recombination directionality of Bxb1 integrase (BBa_K1039003).

In concert with Bxb1 RDF, Bxb1 integrase (BBa_K1039003) catalyzes site-specific recombination between Bxb1 attR and attL sites, to produce Bxb1 attP and attB sites. This recombination is uni-directional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of Bxb1 integrase and RDF.

In concert with Bxb1 RDF (BBa_K1039006), Bxb1 integrase can be used to flip the Bxb1 invertible promoter switches BBa_K1039004 and BBa_K1039005 from RL state to PB state.

Notes

to be written later.

Source

The sequence was designed by Bonnet lab at the Department of Bioengineering, Stanford University, and was further modified by Waterloo team.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Bxb1 RDF	1,768	sequence_feature, feature/misc

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1039006/1

Member of these Collections

• iGEM Parts Registry