| Types | DnaRegion
|
| Roles | Reporter
engineered_region
|
| Sequences | BBa_K1041004_sequence (Version 1)
|
Description
Team NRP-UEA_Norwich 2013 desgined this part using biobricks BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041002 and a gene encoding Gus ligated in front of the promoter of BBa_K1041001 to create a new biobrick.
Notes
Team NRP-UEA_Norwich 2013 desgined this part using biobricks BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041002 and a gene encoding Gus ligated in front of the promoter of BBa_K1041001 to create a new biobrick.
Source
This part was designed by our team to aid our project in identifying new strains of Antimycin-Producing Actinomycetes.The 14 known biosynthetic gene clusters contain four operons: antAB, antCDE, antFG and antHIJKLMNO. The antA gene encodes a unique ECF RNA polymerase sigma factor, referred to as σAntA, which has the sole function of regulating antimycin synthesis by activating transcription of the antFG and antHIJKLMNO genes [1]. Homologues of the AntA sigma factor, the key regulatory protein in antimycin biosynthesis, are present in all known gene clusters [2]. Due to this property a biosensor was designed with the AntA-regulated promoter (antGp) controlling the expression of the reporter Gus. This part was produced by performing restriction digests of the part BBa_K1041001.
