Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Wild, Katja Karstens
Date created: 2008-10-21 11:00:00
Date modified: 2015-05-08 01:08:52
Leu2 - Beta-isopropylmalate dehydrogenase of yeast
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K105016_sequence (Version 1) |
Description
The Beta-isopropylmalate dehydrogenase (IMDH) catalyzes the third step in the leucine biosynthesis pathway of S. cerevisiae. Under the name of Leu2 it is one of the commonly used auxotrophic markers in Yeast. There excists a number of S. cerevisiae strains which are deleated for this enzyme.Our BioBrick contains the protein coding sequence as well as the natural promoter and terminator of the gene. To avoid a transcription of genes downstream of this part, it might be usefull put a strong termintor (i.e. Adh1_term [[Part:BBa_J63002| BBa_J63002]]) in between the parts.
Notes
Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.So there is no base in between the BioBrick and the restriction sites.
For more information about this issus, see:
Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering."
DSpace http://hdl.handle.net/1721.1/32535 (2006).
Source
Amplification from the plasmid pRS315 (Sikorski & Hieter)| Sequence Annotation | Location | Component / Role(s) |
| Beta-isopropylmalate dehydrogenase please mutate A -> G | 660,1755 1298,1298 | feature/cds CDS feature/s_mutation point_mutation |