BBa_K105031

BBa_K105031 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K105031
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Wild
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:08:52

cyc16 minimal promoter



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K105031_sequence (Version 1)

Description

This is a derivate of [[Part:BBa_K105027| BBa_K105027]]. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 16 % of the natural yeast CYC1 promoter.





Notes

This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!



Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.

Source

Amplification and Mutation from the plasmid pAH3

Sequence Annotation Location Component / Role(s)
TATA-box
A -> G
17,23
22,22
sequence_feature feature/misc
point_mutation feature/s_mutation
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K105031/1