BBa_K1059001

BBa_K1059001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1059001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yu Wang
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:08:57

GFP-LVA under J23101 control



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K1059001_sequence (Version 1)

    Description

    This device consists of a GFP with LVA tag under the control of J23101 promoter.

    Notes

    The GFP-LVA is from part K145280, but it is reported that the RBS is so weak that it cannot work well, so we have to change the RBS to B0035 by PCR.

    Source

    The GFP-LVA sequence is from part 145280, but we change the RBS.

    Sequence Annotation Location Component / Role(s)
    J23101
    B0035
    start
    GFP-LVA
    stop codon
    B0015
    		1,35
    46,65
    66,78
    66,824
    818,826
    833,961
    		feature/promoter promoter
    ribosome_entry_site feature/rbs
    feature/start start_codon
    feature/cds CDS
    feature/stop stop_codon
    feature/stem_loop stem_loop

    igem#experience
    Works
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1059001/1
     

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