BBa_K1059004

BBa_K1059004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1059004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Qiu Wang

Date created: 2013-09-16 11:00:00

Date modified: 2015-05-08 01:08:57

Its transcript can prevent the mRNA itself from being degraded by exonuclease.

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1059004_sequence (Version 1)

Description

This coding cosists of a RBS ,a start codon and several stop codons in different reading frames. It can be used in the 3'-UTR of a mRNA, preventing the mRNA degradation mediated by exonuclease. The extra RBS can attract ribosome to bind on it,which can prevent the mRNA from being degraded by exonuclease . The start codon makes binding more efficient and the stop codon just prevents the DNA???s translation.

Notes

The "extra RBS " and the "real RBS " must be enough interval, preventing the ribosome interfe with each other.

Source

This segment is design by oueselves.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
B0035	28,41	feature/rbs, ribosome_entry_site
start codon	48,50	start_codon, feature/start
stop codon	51,56	stop_codon, feature/stop

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1059004/1

Member of these Collections

• iGEM Parts Registry