BBa_K1059006

BBa_K1059006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1059006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xue Sun
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:08:57

DNA segment whose transcript can prevent mRNA degradation by RNaseE in two state .



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1059006_sequence (Version 1)

Description

This coding cosists of a RBS ,a start codon and several stop codons in different reading frames. It can be used in the 5'-UTR of a mRNA, preventing the mRNA degradation mediated by RNaseE.The extra RBS can attract ribosome to bind on it.And it has the ability to prevent the degradosome from being actived by 5' ribonucleotide because of it???s steric hindrance.The start codon makes binding more efficient and the stop codon just prevents the DNA???s translation.

Notes

To make sure the 5' ribonucleotide protected by a ribosome,we must arrange the RBS close to the 5' extreme ribonucleotide.And also ,the "extra RBS " and the "real RBS " must be enough interval, preventing the ribosome interfe with each other.

Source

This segment is design by oueselves.

Sequence Annotation Location Component / Role(s)
RNA thermosensor
J23101
start
stop
100,103
1,34
110,112
119,121
engineered_region feature/BioBrick
feature/promoter promoter
start_codon feature/start
stop_codon feature/stop
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1059006/1