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BBa_K1060003

BBa_K1060003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1060003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ingmar Claes, Robbert Boudewijns, Laurens De Backer

Date created: 2013-09-16 11:00:00

Date modified: 2015-05-08 01:08:57

Methyl Salicylate Production

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Details

Types	DnaRegion
Roles	Generator engineered_region
Sequences	BBa_K1060003_sequence (Version 1)

Description

KU_Leuven 2013 requested the BBa_J45700 brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone. For the assembly of this part we refer to the original MIT brick BBa_J45700 => "We assembled a complete wintergreen odor biosynthetic system (BBa_J45700) by combining the salicylic acid (BBa_J45320) and wintergreen odor generators (BBa_J45120). BBa_J45700 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor."

Notes

no EcoRI, XbaI, NotI, SpeI or PstI sites present

Source

BBa_J45700 (MIT 2006)

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0040 BBa_B0032 BBa_J45004 BBa_B0010 BBa_B0012 BBa_R0011 BBa_B0032 BBa_J45017 BBa_B0010 BBa_B0012	p(tetR) BBa_B0032 BSMT1 BBa_B0010 BBa_B0012 lacI+pL BBa_B0032 pchBA BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040 BBa_B0032 BBa_J45004 BBa_B0010 BBa_B0012 BBa_R0011 BBa_B0032 BBa_J45017 BBa_B0010 BBa_B0012	1,54 63,75 82,1155 1164,1243 1252,1292 1301,1354 1364,1376 1383,3118 3127,3206 3215,3255	p(tetR) BBa_B0032 BSMT1 BBa_B0010 BBa_B0012 lacI+pL BBa_B0032 pchBA BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	In stock
igem#status	Available
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K1060003/1