Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ingmar Claes
Date created: 2013-09-23 11:00:00
Date modified: 2015-05-08 01:08:58
LacI-AroG* production under regulation of TetR repressible promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1060013_sequence (Version 1) |
Description
Regulatory construct to get expression of LacI (BBa_C0012) and mutated AroG (Bba_K1060000) under the control of the TetR repressible promoter (Bba_R0040). The mutated AroG (Pro150Leu and Leu175Asp) is less repressed by phenylalanine resulting in a upscaling of the shikimate pathway and production of more chorismic acid (ChA). ChA being a precursor of methyl salicylate. A biosensor which expresses TetR (C0040) under the certain conditions could switch of LacI and mutated AroG expression.Notes
no EcoRI, XbaI, NotI, SpeI or PstI sites presentSource
Escherichia coli genome K12 (DH5alpha)| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_B0030 BBa_C0012 BBa_B0032 AroG* (mutated) BBa_K823017 | 1,54 61,75 82,1234 1243,1255 1262,2314 2323,2417 | engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick feature/BioBrick engineered_region feature/cds CDS engineered_region feature/BioBrick |