Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Horwitz
Date created: 2008-10-28 12:00:00
Date modified: 2015-05-08 01:09:00
AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K106697_sequence (Version 1) |
Description
This is a variant of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t), with the addition of 8X LexA operator sites directly downstream of the Adht1 terminator.Notes
None.Source
Variant of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t)| Sequence Annotation | Location | Component / Role(s) |
| CEN ARS ampicillin resistance marker Cyc1 Promoter D overhang Adh1 terminator 8X LexA Operators LEU2 marker T7 promoter lacZalpha reporter f1 origin ampicillin resistance promoter pBR322 origin lac promoter T3 promoter Stop codons in all 3 frames Start codon A overhang | 3565,4083 4215,5075 6294,6556 1,4 30,209 210,377 1732,2838 386,404 408,567 589,895 4145,4173 5230,5849 6158,6187 6257,6276 11,21 6547,6549 6556,6559 | feature/misc sequence_feature CDS feature/cds feature/promoter promoter sequence_feature feature/dna stem_loop feature/stem_loop operator feature/operator feature/cds CDS promoter feature/promoter sequence_feature feature/misc sequence_feature feature/dna feature/promoter promoter sequence_feature feature/dna promoter feature/promoter feature/promoter promoter feature/stop stop_codon start_codon feature/start feature/dna sequence_feature |