Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Junqi Kuang, Yaqing du, Xihao liao and Junjie zhang
Date created: 2013-09-07 11:00:00
Date modified: 2015-05-08 01:09:01
Pgpa1+Chimeric Gpa1(Gα
subunit)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1072011_sequence (Version 1) |
Description
In yeast, the pheromone receptors are coupled to a heterotrimeric G protein. This is composed of Gpa1p (Gα), Ste4p (Gβ) and Ste18p(Gγ) subunits. In order to coupling heterologous GPCRs in yeast successfully, ???Transplants??? have the five C-terminal residues of Odr3 of C. elegans, replaced with the corresponding mammalian Gα residues(Brown et al. 2000).Notes
-Source
Originate from C. elegans| Sequence Annotation | Location | Component / Role(s) |
| pGpa1 Gpa1 Coding region | 1,1000 1001,2419 | feature/promoter promoter feature/cds CDS |