| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1076005_sequence (Version 1)
|
Description
Gene of fatty acid transport protein, outer membrane; long-chain fatty acid receptor. We wanted this ORF gene in order to enhance the long chain fatty acids intake by Shewanella putrefaciens to increase B-oxidation and therefore ATP and H+ production.Must be completed with promoter, RBS and terminator.
Notes
We chosed the ORF of this gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3. Further subclonning and expression should be done within pSB3C5. Should also be assembled with promoter, RBS and terminator.
Source
From Genomic DNA from Shewanella putrefaciens
