Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Macquarie University
Date created: 2013-09-21 11:00:00
Date modified: 2015-05-08 01:09:03
GUN4 (+ Ptac Promoter)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1080013_sequence (Version 1) |
Description
Tetrapyrrole-binding protein - In Arabidopsis, GUN4 (Genomes uncoupled 4) is required for the functioning of the plastid mediated repression of nuclear transcription that is involved in controlling the levels of magnesium- protoporphyrin IX. GUN4 binds the product and substrate of Mg-chelatase, an enzyme that produces Mg-Proto, and activates Mg-chelatase. GUN4 is thought to participates in plastid-to-nucleus signaling by regulating magnesium-protoporphyrin IX synthesis or trafficking. This composite part contains the promoter BBa_K864400. The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1].[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
Notes
Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence. Transformation done using Dh5-alpha competent cells.Source
Promoter - Created from oligos ordered from SigmaAldrich.Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA
Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG
GUN4 - Chlamydomonas reinhardtii
| Access | Instance | Definition |
| public public | BBa_K864400 BBa_K1080003 | BBa_K864400 "The Gun" |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K864400 BBa_K1080003 | 1,61 70,797 | BBa_K864400 "The Gun" |