BBa_K1085009

BBa_K1085009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1085009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Claudio Tiecher

Date created: 2013-09-09 11:00:00

Date modified: 2015-06-02 12:11:00

SilkTail Strep-tag Stop-codon

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K1085009_sequence (Version 1)

Description

This BioBrick contains the coding sequence for silk tail with a Strep-tag behind it and ends with a stop codon.
The Tail ??????.

The Strep-tag, fused at the C terminal, is used both as a binding tag to use to inidicate its production and as a binding component to biotin.

Notes

The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.

Source

The amino acid sequence of the tail was obtained from MaSp2. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
strep	289,312	tag, feature/tag
spidersilk C-terminal domain	1,270	feature/cds, CDS
Stop codon (TGA)	313,315	stop_codon, feature/stop

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1085009/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //proteindomain/tail
• iGEM Parts Registry