Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ting-Yun Chiang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:09
AhpCpD1
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1104206_sequence (Version 1) |
Description
- We improve the function of a BioBrick Part: AhpC promoter(K362001)designed by 2010 KIT-Tokyo team into four versions.On PartRegistry, the complex part(according to Ecocyc) composition contains hybrid promoters, shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG.
- OxyR binding to the ahpC-proximal site leads to the induction of both dsbG and ahpC transcripts, while OxyR binding to the dsbG-proximal site leads to the induction of a second ahpC transcript. This transcript of ahpC and the transcript of dsbG overlap by over 100 nucleotides.
- The intergenic region between dsbG and ahpC carries two binding sites for OxyR, a dsbG-proximal site, located 54 bp upstream of the dsbG start codon, and an ahpC-proximal site, located 290 bp upstream of the dsbG start codon.
Notes
.Source
E. coli MG1655 genomic sequence| Sequence Annotation | Location | Component / Role(s) |
| OxyR binding site3 OxyR binding site4 (AhpCp1)-35 (AhpCp1)-10 DsbGp | 72,88 94,110 109,114 133,138 1,25 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/promoter promoter feature/promoter promoter promoter feature/promoter |