BBa_K1113401

BBa_K1113401 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1113401
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Belen Cespedes
Date created: 2013-09-17 11:00:00
Date modified: 2015-05-08 01:09:11

Targeting sequence for the delivery of the LacZ gene to the Carboxysome



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K1113401_sequence (Version 1)

Description

Includes the lacZ gene (BBa_I732005) which encodes for the β-galactosidase attached with a linker (BBa_K105012) to the large subunit of the RuBisCo (BBa_K1113701) under the regulation of the pBad/araC promoter (BBa_I0500) and includes a RBS (BBa_B0034). The construct is for the delivery of the β-galactosidase and test reactions within the carboxysome.
Source of this part: The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley)

Notes

This part it constructed with a mutation in the Prefix (from ???cggccgc??? to ???atacctg???) which erase the NotI restriction site, this modification allow the correct design of primers for Gibson assembly

Source

The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley)

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It's complicated
igem#status
Planning
 
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user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1113401/1