| Types | DnaRegion
|
| Roles | engineered_region
Reporter
|
Description
GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag.
Notes
-
Source
This part consist in the superfolder GFP (BBa_I746916) attached to two targeting sequences: The large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme which deliver into the carboxysome and the ssrA degradation tag which is a targeting sequence for degradation by the protease ClpXP. This construct is used as a reporter for the correct assembly of the Carboxysome, it works with the competition of the two targeting sequence, in the case of a correct assembly of the carboxysome the microcompartment will ???protect??? the fluorescens, in any other case this will be degraded by the protease.
