BBa_K1114400

BBa_K1114400 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1114400
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Devina Desai
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:12

This is a MoClo level 0 destination vector.



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1114400_sequence (Version 1)

Description

This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites A on the 5' side and site B on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Summary of modifications from original part:


Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.

Notes

This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites A on the 5' side and site B on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Summary of modifications from original part:


Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.

Source

iGEM kit

Sequence Annotation Location Component / Role(s)
BsaI
BsaI
SpeI
SpeI
SpeI
MoClo Fusion Site A
MoClo Fusion Site B
alpha fragment of lacZ
BbsI
BbsI
14,19
528,533
1,6
7,12
535,540
21,24
523,526
33,514
27,32
515,520
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
CDS feature/cds
feature/misc sequence_feature
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1114400/1