Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wai In Hui
Date created: 2013-09-10 11:00:00
Date modified: 2015-05-08 01:09:13
Mitochondrial Leader Sequence with RFC25 standard
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_K1119001_sequence (Version 1) |
Description
Mitochondrial leader sequence (MLS) helps direct protein to the mitochondria. When this sequence is cloned in frame at the N-terminus of the protein, it can target the recombinant protein to the mitochondria in mammalian cells. MLS will be removed upon translocation into the mitochondrial matrix. However, at least four additional amino acids (Ile-His-Ser-Leu) on this sequence are known to be retained on the N-terminus of the protein.Since MLS always fuses with protein for function, this BioBrick is in RFC 25 standard, which facilitate protein fusion in order to introduce other protein into mitochondria.
Notes
NoSource
Invitrogen: pCMV/myc/mito (http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf)| Sequence Annotation | Location | Component / Role(s) |
| Mitochondrial Leader Sequence | 1,87 | feature/protein CDS |