Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-09-02 11:00:00
Date modified: 2015-05-08 01:09:13
rbs.T4 holin!
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K112005_sequence (Version 1) |
Description
T4 holin gene with a native rbs and a stop codon, in BBb format.This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
BBb Standard Description Page
Notes
N/ASource
PCR bx6/bx2 on Bjh1301 (704 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112005
bx6 Forward Biobricking of {rbs.T4 Holin!}
ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
bx2 Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc