Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-09-02 11:00:00
Date modified: 2015-05-08 01:09:13
T4 antiholin! in BBb
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K112006_sequence (Version 1) |
Description
T4 antiholin gene with start and stop codons, in BBb format.This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
BBb Standard Description Page
Notes
N/ASource
PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112006
bx7 Forward biobricking of {T4 Antiholin!}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx8 Reverse biobricking of {T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc