BBa_K112007

BBa_K112007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-09-02 11:00:00
Date modified: 2015-05-08 01:09:13

< T4 antiholin! in BBb



    • Details

    Types
    DnaRegion

    Roles
    Protein_Domain

    polypeptide_domain

    Sequences BBa_K112007_sequence (Version 1)

    Description

    T4 antiholin gene with a start codon, with a stop codon, in BBb format.

    This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Notes

    N/A

    Source


    
PCR bx9/bx8 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
    
Sub into pBca1256			(EcoRI/BamHI)
    
Product is pBca1256-K112007
    


    
bx9		Forward biobricking of {
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
    
bx8		Reverse biobricking of {
cgttaGGATCCtcattcagtctccaatttaatgttc


    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K112007/1
     

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