Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-09-02 11:00:00
Date modified: 2015-05-08 01:09:13
T4 antiholin> in BBb
| Types | DnaRegion |
| Roles | Protein_Domain polypeptide_domain |
| Sequences | BBa_K112008_sequence (Version 1) |
Description
T4 antiholin gene with a start codon, without a stop codon, in BBb format.This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
BBb Standard Description Page
Notes
N/ASource
PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112008
bx7 Forward biobricking of {T4 Antiholin>}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx10 Reverse biobricking of {T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc