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BBa_K112012

BBa_K112012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-09-02 11:00:00

Date modified: 2015-05-08 01:09:13

T4 lysozyme! in BBb

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Details

Types	DnaRegion
Roles	CDS Coding
Sequences	BBa_K112012_sequence (Version 1)

Description

T4 lysozyme gene with start and stop codons, in BBb format.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source



PCR bx13/bx14 on Bjh1302		(526 bp, EcoRI/BamHI/DpnI)

Sub into pBca1256				(EcoRI/BamHI)

Product is pBca1256-K112012



bx13      Forward biobricking of {T4 Endolysin!}

ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg

bx14      Reverse biobricking of {T4 Endolysin!}

cgttaGGATCCttatagatttttatacgcgtcccaagtgcc