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BBa_K112013

BBa_K112013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-09-02 11:00:00

Date modified: 2015-05-08 01:09:13

< T4 lysozyme! in BBb

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Details

Types	DnaRegion
Roles	Protein_Domain polypeptide_domain
Sequences	BBa_K112013_sequence (Version 1)

Description

T4 lysozyme gene without a start codon, with a stop codon, in BBb format.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source



PCR bx15/bx14 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)

Sub into pBca1256				(EcoRI/BamHI)

Product is pBca1256-K112013



bx15      Forward biobricking of {
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg

bx14      Reverse biobricking of {
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K112013/1