BBa_K112103

BBa_K112103 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112103

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Madhvi Venkatesh

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:14

BamHI endonuclease

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K112103_sequence (Version 1)

Description

BamHI enzyme with start and stop codons

Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

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PCR mv005/mv006 on pBca9145-I716210    (673bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112103



mv005	Forward Biobricking of r.BamH1!

ccataGAATTCatgAGATCTatggaagtagaaaaagagtttattactgatgaagc

mv006	Reverse Biobricking of r.BamH1!

cgttaGGATCCctatttgttttcaactttatctttcc

Source

PCR mv005/mv006 on pBca9145-I716210    (673bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112103

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112103/1

Member of these Collections

• iGEM Parts Registry