Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:14
Ligase with a start codon without a stop codon
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_K112112_sequence (Version 1) |
Description
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
BBb Standard Description Page
Source
PCR mv021/mv023 on pBca1256-K112109 (1492bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112112
mv021 Forward Biobricking of ligase!
ccataGAATTCatgAGATCTatgattcttaaaattctgaacg
mv023 Reverse Biobricking of ligase>
cgttaGGATCCtagaccagttacctcatg